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chemidocmp imaging system  (Bio-Rad)


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    Structured Review

    Bio-Rad chemidocmp imaging system
    Chemidocmp Imaging System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 22869 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chemidocmp+imaging+system/ChemiDoc+MP+Imaging+System/10__3390_slash_pathophysiology33020032-130-5-8
    Average 99 stars, based on 22869 article reviews
    chemidocmp imaging system - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Western Blot:

    Article Title: ARID1A deficiency unleashes centromeric RNA transcription to drive chromosomal instability and boosts PKMYT1 inhibitor efficacy via RNA sensing
    Article Snippet: .. The western blots were visualized using Clarity Western ECL substrate (Bio-Rad 170–5061) and scanning with a ChemiDocMP imaging system (Bio-Rad). .. Total RNAs (1ug) were purified with PureLink RNA mini kit (#12183018A, Thermo Fisher Scientific), then reverse transcribed into cDNA by using HiScript IV RT SuperMix (#R423-01, Vazyme).

    Article Title: Covalent modification of a glutamic acid inspired by HaloTag technology.
    Article Snippet: For detection of PDEδ, loading control β-actin, aswell as phosphorylated S6 ribosomal protein (2F9) (pS6P on S235/S236) and total S6 ribosomal protein (54D2) (tS6P), membranes were blocked with 5% milk in PBS-T for 1 h at room temperature, followed by washing, incubation with the primary antibody, anti-PDEδ (Invitrogen, PA5-22008, RRID: AB_11154288, 1:500), or anti-β-actin (Abcam, ab8227, RRID:AB_2305186, 1: 5000), or anti-pS6P (Cell Signalling Technology, #4856, RRID: AB_2181037, 1:1000), or anti-tS6P (Cell Signalling Technology, #2317, RRID: AB_2238583, 1:500) overnight at 4 °C and subsequently with horseradish peroxidase (HRP)conjugated secondary antibodies (Invitrogen, #62-6520, RRID: AB_88369 for anti-mouse, 1:5000; Invitrogen, #31460, RRID: AB_228341 for anti-rabbit, 1:5000) for 1 h at room temperature. .. After washing with PBS-T five times, secondary antibody-incubated membranes were imaged with the ChemiDocMP Imaging System (BIO-RAD Nature Communications | (2026) 17:1257 15 Laboratories) directly with the IRDye800CW channel for visualisation of vinculin bands, or with the addition of western blotting detection reagent (SuperSignalTM West Dura Extended Duration Substrate, Thermo Fisher Scientific, #34075) for the HRP conjugated systemwith chemiluminescence channel. .. Relative band intensities were quantified using Image Lab (BIO-RAD).

    Imaging:

    Article Title: ARID1A deficiency unleashes centromeric RNA transcription to drive chromosomal instability and boosts PKMYT1 inhibitor efficacy via RNA sensing
    Article Snippet: .. The western blots were visualized using Clarity Western ECL substrate (Bio-Rad 170–5061) and scanning with a ChemiDocMP imaging system (Bio-Rad). .. Total RNAs (1ug) were purified with PureLink RNA mini kit (#12183018A, Thermo Fisher Scientific), then reverse transcribed into cDNA by using HiScript IV RT SuperMix (#R423-01, Vazyme).

    Article Title: Eukaryote initiation factor 6 modulates small-cell lung carcinoma plasticity via the integrin-FAK signaling axis.
    Article Snippet: .. Themembranes were finally scanned using the ChemiDocMP imaging system (Bio-Rad) and analysed using Image Lab (Bio-Rad). ..

    Article Title: The Action Mechanism of Dendrobium spp. in Treating Dry Eye Disease Based on Network Pharmacology and In Vitro Experiments
    Article Snippet: Protein bands were visualized using an enhanced chemiluminescence (ECL) detection kit (Thermo Fisher Scientific, Inc., Waltham, MA, USA; Cat No. 34580). .. The chemiluminescent signals were captured using a ChemiDocMP Imaging System (Bio-Rad Laboratories, Inc., Hercules, CA, USA). and analyzed using the ImageJ software (version 1.53; National Institutes of Health, Bethesda, MD, USA). ..

    Article Title: Pantothenic Acid Derivatives Modulate Oxidative Stress and Hepatic Fibrosis in Bile Duct Ligation-Induced Cholestatic Liver Injury
    Article Snippet: The resulting chemiluminescent signal was detected using a WesternBright ECL kit (Advansta, San Jose, CA, USA). .. Visualization was performed using a ChemiDocMP imaging system (Bio-Rad, Hercules, CA, USA). .. To confirm protein loading after electrophoresis, TGX Stain-Free imaging technology (Bio-Rad, Hercules, CA, USA) was used according to the manufacturer’s instructions.

    Article Title: Covalent modification of a glutamic acid inspired by HaloTag technology.
    Article Snippet: For detection of PDEδ, loading control β-actin, aswell as phosphorylated S6 ribosomal protein (2F9) (pS6P on S235/S236) and total S6 ribosomal protein (54D2) (tS6P), membranes were blocked with 5% milk in PBS-T for 1 h at room temperature, followed by washing, incubation with the primary antibody, anti-PDEδ (Invitrogen, PA5-22008, RRID: AB_11154288, 1:500), or anti-β-actin (Abcam, ab8227, RRID:AB_2305186, 1: 5000), or anti-pS6P (Cell Signalling Technology, #4856, RRID: AB_2181037, 1:1000), or anti-tS6P (Cell Signalling Technology, #2317, RRID: AB_2238583, 1:500) overnight at 4 °C and subsequently with horseradish peroxidase (HRP)conjugated secondary antibodies (Invitrogen, #62-6520, RRID: AB_88369 for anti-mouse, 1:5000; Invitrogen, #31460, RRID: AB_228341 for anti-rabbit, 1:5000) for 1 h at room temperature. .. After washing with PBS-T five times, secondary antibody-incubated membranes were imaged with the ChemiDocMP Imaging System (BIO-RAD Nature Communications | (2026) 17:1257 15 Laboratories) directly with the IRDye800CW channel for visualisation of vinculin bands, or with the addition of western blotting detection reagent (SuperSignalTM West Dura Extended Duration Substrate, Thermo Fisher Scientific, #34075) for the HRP conjugated systemwith chemiluminescence channel. .. Relative band intensities were quantified using Image Lab (BIO-RAD).

    Article Title: Identification and validation of a sphingolipid metabolism-related prognostic signature for predicting prognosis and immune microenvironment-related characteristics in ovarian cancer
    Article Snippet: After washing three times with TBST, the membrane was incubated at room temperature for 2 h with HRP-conjugated secondary antibody diluted in secondary antibody dilution buffer (cat. no. P0023D, Beyotime, Shanghai, China). .. Finally, protein bands were visualized using ECL substrate (cat. no. 36208ES76, Yeasen, Shanghai, China) and imaged with the Tanon-4600 chemiluminescence imaging system (Tanon, Shanghai, China) and the ChemiDocMP imaging system (Bio-Rad, Hercules, CA, USA). .. The following antibodies were used: CERK polyclonal antibody (cat. no. 25731-1-AP, Proteintech, Wuhan, China) (900 μg/mL, diluted 1:1,000), Recombinant Anti-beta Actin antibody (HRP Conjugated) (cat. no. ZB15001-HRP, Servicebio, Wuhan, China) (diluted 1:1,000, used as loading control), HRP-conjugated Goat Anti-Rabbit IgG (H+L) (cat. no. GB23303, Servicebio, Wuhan, China) (diluted 1:10,000).



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